01 October 2010
The Role of Candida in inflammatory bowel disease. Estimation of transmission of C. albicans fungi in gastrointestinal tract based on genetic affinity between strains
Danuta TrojanowskaABCDEF, Malgorzata Zwolinska-WcisloBDG, Marianna TokarczykCF, Krzysztof KosowskiBD, Tomasz MachDG, Alicja BudakACDMed Sci Monit 2010; 16(10): CR451-457 :: ID: 881193
Abstract
Background: The pathogenesis of inflammatory bowel disease (IBD), is related to the effect of fungal presence in the gastrointestinal tract (GI) on IBD development.
Material/Methods: One hundred twenty-six patients underwent mycological investigation. We analyzed of the occurrence of fungal infection in the clinical material collected from the GI tract of patients with IBD and control groups. We assessed fungal flora transmission from the oral cavity to further segments of the GI tract based on comparative studies of genotypes of fungal strains. The studies were carried out using conventional methods. The fungal genotypes were determined with the PCR-RAPD method.
Results: Of 407 samples, 220 of them had fungi. The most frequently isolated species was C. albicans in patients with IBD and in control group, respectively 84.4% and 81%. Analysis of the streak patterns of the products of the PCR-RAPD reaction carried out for C. albicans strains isolated from the material collected from 10 patients, showed 100% affinity between the strains cultivated from the same patient. Genetic affinity between the strains was confirmed in 3 primers used in the examination.
Conclusions: The presence of fungi in the oral cavity of patients with IBD may affect more frequent colonization of the colonic mucosa in the active phase of disease. Genetic affinity of C. albicans strains indicates the possibility of fungal transmission from the oral cavity to further segments of the GI tract.
Keywords: Mouth - microbiology, Intestinal Mucosa - microbiology, Inflammatory Bowel Diseases - microbiology, Gastrointestinal Tract - microbiology, Fungi - isolation & purification, Crohn Disease - microbiology, Colitis, Ulcerative - microbiology, Case-Control Studies, Candida - isolation & purification, Adolescent, Mycoses - microbiology, Polymerase Chain Reaction - methods, Random Amplified Polymorphic DNA Technique - methods, young adult
Editorial
01 May 2024 : Editorial
Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)DOI: 10.12659/MSM.944927
Med Sci Monit 2024; 30:e944927
In Press
12 Mar 2024 : Clinical Research
Preoperative Blood Transfusion Requirements for Hemorrhoidal Severe Anemia: A Retrospective Study of 128 Pa...Med Sci Monit In Press; DOI: 10.12659/MSM.943126
12 Mar 2024 : Clinical Research
Tissue Inhibitors of Metalloproteinase 1 (TIMP-1) and 3 (TIMP-3) as New Markers of Acute Kidney Injury Afte...Med Sci Monit In Press; DOI: 10.12659/MSM.943500
12 Mar 2024 : Review article
Optimizing Behçet Uveitis Management: A Review of Personalized Immunosuppressive StrategiesMed Sci Monit In Press; DOI: 10.12659/MSM.943240
12 Mar 2024 : Clinical Research
Metabolomic Alterations in Methotrexate Treatment of Moderate-to-Severe PsoriasisMed Sci Monit In Press; DOI: 10.12659/MSM.943360
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952