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Karolina Kłoda, Leszek Domański, Ewa Kwiatkowska, Krzysztof Safranow, Arleta Drozd, Andrzej Ciechanowicz, Kazimierz Ciechanowski
(Clinical Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University in Szczecin, Szczecin, Poland)
Ann Transplant 2016; 21:392-399
Immunosenescence is closely linked to chromosome telomere erosion and telomerase activity alterations. The aim of this study was to analyze the associations of relative telomere length (RTL) of a graft with sex, comorbid conditions, post-transplant infections, and CMV reactivation among transplanted kidney recipients. Additionally, the associations of donor and recipient hTERT, BICD1 genes and chromosome 18 polymorphisms with post-transplant infections were analyzed, including the analysis of donor-recipient genotype pairs.
MATERIAL AND METHODS: The study enrolled 119 white Polish kidney allograft recipients (64M/55F, mean age 47.3±14.0). The RTL was assessed by modification of a method developed by Cawthon, using a qPCR system. To identify genotypes of the studied polymorphisms, real-time PCR was performed.
RESULTS: There were no significant associations between graft RTL and sex of donor and recipient, comorbid DM and AH, as well as post-transplant infections and CMV reactivation. There were no statistically significant differences in distribution of hTERT, BICD1 genes and chromosome 18 graft and recipient polymorphisms genotypes between individuals with post-transplant infection and those without infection. The rs2735940 CX-TT hTERT gene donor-recipient genotypes combination was associated with higher risk of post-transplant infection on the border of statistical significance (OR=4.632, 95%CI (0.853–25.14); p=0.067).
CONCLUSIONS: Assessment of kidney allograft RTL does not show its association with sex, DM, AH, post-transplant infection, or CMV reactivation in the recipients, suggesting that other factors, probably directly related to the transplantation procedure, have a greater effect on telomere length.
Keywords: Allografts, Cytomegalovirus Infections, Polymorphism, Single Nucleotide, Telomere Homeostasis