22 December 2020>: Original Paper
Circulating NKG2A–NKG2D+ CD56dimCD16+ Natural Killer (NK) Cells as Mediators of Functional Immunosurveillance in Kidney Transplant Recipients
Li Zhu 12ABCDEF , Hristos Karakizlis 3B , Rolf Weimer 3B , Christian Morath 4B , Naruemol Ekpoom 2B , Eman H. Ibrahim 2BE , Gerhard Opelz 2ADEF , Volker Daniel 2ABCDEF*DOI: 10.12659/AOT.925162
Ann Transplant 2020; 25:e925162
Table 2 Antibody panel for flow cytometric tests of peripheral blood lymphocytes.
V450 | V500 | FITC | PE | PercpCy5.5 | PE-Cy7 | APC | APC-Cy7 | |
---|---|---|---|---|---|---|---|---|
IgG | IgG | |||||||
CD158a | CD158e | |||||||
NKG2D | NKG2A | |||||||
IL-4R | CD158b | |||||||
CD69 | CD107a | |||||||
CD119 | CD25 | |||||||
TGFβRII | IL-10R | |||||||
cIL-10 | NKG2A | cINFγ | ||||||
cIL-4 | NKG2A | cTGFβ | ||||||
cPerforin | NKG2A | cGranzymeB | ||||||
cIL17 | CD4 | CD8 | ||||||
Foxp3 | CD127 | CD25 | ||||||
cINFγ | Foxp3 | CD127 | CD25 | |||||
Table shows the monoclonal antibody panel with the corresponding fluorochromes. Monoclonal antibodies in italic font were used in every tube whereas monoclonal antibodies written in regular font were only used in certain tubes. |