17 February 2017 : Original article
Effects of Static Cold Storage and Hypothermic Machine Perfusion on Oxidative Stress Factors, Adhesion Molecules, and Zinc Finger Transcription Factor Proteins Before and After Liver TransplantationDe-Fang Zhao1BCDEF, Qin Dong1BCDEF, Tong Zhang1ABCDG*
Ann Transplant 2017; 22:96-100
BACKGROUND: This study aimed to investigate the effects of static cold storage (SCS) and hypothermic machine perfusion (HMP) on the oxidative stress factors (OSF), adhesion molecules (AM), and zinc finger transcription factor (Snail) before and after liver transplantation.
MATERIAL AND METHODS: Experimental dogs were randomly divided into donor (group A), SCS (group B), and HMP (group C) (n=30) groups. Livers retrieved from group A were transplanted into group B after SCS, and the livers sampled from group B were transplanted into group C after HMP. The dogs in group A were euthanized and discarded, and the livers sampled from group C were used for other experiments. Twenty dogs with successful liver transplants were randomly selected from groups B and C for analysis.
RESULTS: During the liver sampling process, the levels of OSF, AM, and Snail between the 2 groups showed no significant differences (P>0.05); before the transplantation, the levels of chemokine CXCL14 and Snail between the 2 groups showed no significant differences (P>0.05), and compared with group B, HIF-1α and P-selectin in group C were lower (P<0.01); 60 min after the transplantation, HIF-1α, chemokine CXCL14, P-selectin, and Snail in group C were lower than that in group B (P<0.01).
CONCLUSIONS: HMP can significantly reduce the levels of OSF and inflammatory factors, which is conducive for liver transplantation.
Keywords: Cell Adhesion Molecules, Liver Transplantation, Transplantation, Heterologous
11 Jan 2022 : Original articleEfficacy of Nitric Oxide-Releasing Nanofibers in Reducing Renal Ischemia-Reperfusion Injury in a Rat Model
Ann Transplant In Press; DOI: 10.12659/AOT.934800
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