Association of the rs10918594 of Nitric Oxide Synthase 1 Adaptor Protein (NOS1AP) polymorphisms with the graft function after kidney transplantation
Maciej Zukowski, Katarzyna Kotfis, Jowita Biernawska, Malgorzata Zegan-Baranska, Wojciech Blaszczyk, Mariusz Kaczmarczyk, Andrzej Ciechanowicz, Agnieszka Binczak-Kuleta, Miroslaw Brykczynski, Zbigniew Zietek, Romuald Bohatyrewicz
Ann Transplant 2011; 16(3): 72-76
Background: The purpose of this study was to investigate the relationship of nitric oxide synthase 1 adaptor protein (NOS1AP) polymorphism with serum creatinine level and occurrence of delayed graft function (DGF) in kidney transplant recipients.
Material/Methods: This prospective observational study included 75 kidney transplantations. The data from 40 kidney donors (8 females, 32 males) included sex, age, and cause of death. Donors fully met multi-organ transplantation criteria. Recipient data included sex, age, cause of renal insufficiency, and time and number of hemodialyses prior to transplantation. Applying polymerase chain reaction restriction fragments length polymorphism method, we investigated rs10918594 NOS1AP polymorphism among the 75 kidney recipients. The function of every transplanted kidney was correlated with this polymorphism. We defined DGF as requirement for at least 1 hemodialysis after kidney transplantation. We investigated the association of NOS1AP polymorphism with the recipient serum creatinine levels at day 1 and 180 days after kidney transplantation and the occurrence of DGF.
Results: The analysis of variance showed higher serum creatinine levels in kidney recipients with GG genotype compared with the CC_CG genotype at day 1 and at day 180 post-transplantation. Occurrence of DGF in the post-operative period in kidney recipients with the variant genotypes CG compared with the GG_CC genotype was higher, although these differences were no statistically significant.
Conclusions: Our data suggests no statistically significant association of the rs10918594 polymorphism of nitric oxide synthase 1 adaptor protein (NOS1AP) with the graft function after kidney transplantation.
Keywords: renal transplantation, NOS1AP polymorphism, Delayed Graft Function