Lack of association of C599T polymorphism in the glutathione peroxidase (GPX1) gene with delayed graft function, acute kidney graft rejection and chronic allograft nephropathy
Grazyna Dutkiewicz, Agnieszka Binczak-Kuleta, Andrzej Pawlik, Krzysztof Safranow, Magda Wisniewska, Andrzej Ciechanowicz, Violetta Dziedziejko, Kazimierz Ciechanowski, Leszek Domanski
Ann Transplant 2010; 15(3): 30-34
Background: The glutathione peroxidase/glutathione system is a major defense in oxidative stress. Glutathione peroxidase (GPx) is a selenium-containing antioxidant enzyme that effectively reduces hydrogen peroxide and lipid peroxides to water and lipid alcohols, respectively, and in turn oxidizes glutathione to glutathione disulfide. Previous studies have shown that the activity of glutathione peroxidase is genetically determined and is associated with polymorphisms in GPX1 gene. The aim of the present study was to examine the association between the C599T polymorphism in the glutathione peroxidase (GPX1) gene and delayed graft function of kidney allografts, acute rejection and chronic allograft nephropathy.
Material/Methods: One hundred eighty-seven recipients of first cadaveric renal transplants from the Department of Nephrology, Transplantology and Internal Medicine of Pomeranian Medical University were included in this retrospective study. Genotyping of C599T polymorphism in the GPX1 gene was performed using PCR-RFLP method.
Results: There were no significant associations between this polymorphism and delayed graft function, acute rejection and chronic allograft nephropathy.
Conclusions: The present results suggest that GPX1 C599T polymorphism has no influence on the graft function in the first phase after transplantation, as well as on the acute kidney graft rejection and chronic allograft nephropathy.
Keywords: acute rejection, Chronic Allograft Nephropathy, Delayed Graft Function, Glutathione Peroxidase, Polymorphism