Logo Annals of Transplantation Logo Annals of Transplantation Logo Annals of Transplantation

21 May 2009

Molecular epidemiology of vancomycin resistant enterococcus faecalis amongst patients of transplantology and transplantation surgery wards

G Młynarczyk, W Grzybowska, A Mrówka, S Tyski, T Buczkowska, J Pazik, M Durlik, A Kwiatkowski, L Adadyński, A Chmura, L Paczek, A Młynarczyk

Ann Transplant 2009; 14(1): 70-70 :: ID: 880458

Abstract

Background: Although amongst vancomycin resistant (VR) enterococci (VRE) isolated from patients of Transplantation Institute Enterococcus faecinum predominated, some isolates of Enterococcus faecalis still occurred. The aim of our work was to perform molecular analysis of VR-E. faecalis strains derived from different years.
Material/Methods: Strains of VR E. faecalis were defrosted, multiplied and their DNAs were examined for the presence of vanA, vanB, vanD or vanG genes by PCR. The species status of each strain was confirmed by demonstration of the presence of the ddl gene specific for E. faecalis. PFGE was performed on bacterial DNA digested with SmaI. Products of digestion were separated using CHEF-DR III (Bio-Rad) and the obtained patterns of bands were compared.
Results: In 2003-2006 12 strains VR E. faecalis from 8 patients were isolated.
Two patients had liver transplanted and six kidney. All examined strains harboured vanA gene. Among investigated strains, five displayed patterns very similar to each other, even though they were isolated in different years and from different patients (2 in 2003, 1 in 2004 and 2 in 2005). All 5 strains were susceptible to ampicillin and highly resistant to aminoglycosides (HLAR). The patterns of bands after PFGE in the case of strains from remaining three patients were unrelated.
Conclusions: The results suggested also that the single strain of VR-E. faecalis
was present for three years in closely related hospital wards. The strain disappeared in 2006 and 2007.

Add Comment 0 Comments

In Press

08 Mar 2024 : Original article  

Association of Coronary Calcium Score on Cardiac PET During Pre-Kidney Transplant Assessment with Persisten...

Ann Transplant In Press; DOI: 10.12659/AOT.943532  

14 Mar 2024 : Original article  

Impact of Blood Products Transfusion on Patients in the Immediate Post-Lung Transplant Period: A Cohort Study

Ann Transplant In Press; DOI: 10.12659/AOT.943652  

14 Mar 2024 : Case report  

Treatment of Cavernous Transformation of Portal Vein Caused by Hepatic Cystic Echinococcosis Using Ex Vivo ...

Ann Transplant In Press; DOI: 10.12659/AOT.942358  

15 Mar 2024 : Review article  

Approaches and Challenges in the Current Management of Cytomegalovirus in Transplant Recipients: Highlighti...

Ann Transplant In Press; DOI: 10.12659/AOT.941185  

Most Viewed Current Articles

05 Apr 2022 : Original article  

Impact of Statins on Hepatocellular Carcinoma Recurrence After Living-Donor Liver Transplantation

DOI :10.12659/AOT.935604

Ann Transplant 2022; 27:e935604

12 Jan 2022 : Original article  

Risk Factors for Developing BK Virus-Associated Nephropathy: A Single-Center Retrospective Cohort Study of ...

DOI :10.12659/AOT.934738

Ann Transplant 2022; 27:e934738

22 Nov 2022 : Original article  

Long-Term Effects of Everolimus-Facilitated Tacrolimus Reduction in Living-Donor Liver Transplant Recipient...

DOI :10.12659/AOT.937988

Ann Transplant 2022; 27:e937988

15 Mar 2022 : Case report  

Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...

DOI :10.12659/AOT.935860

Ann Transplant 2022; 27:e935860

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358