21 May 2009
Myocardial fibrosis and cardiocyte diameter are the most important factors discriminating histostructure of dilatative and ischemic cardiomyopathy – study of hearts explanted from transplant recipient
J Nożyński, M Zakliczyński, D Konecka-Mrówka, E Zembala-Nożyńska, D Lange, K Dąbrówka, T Męcik-Kronenberg, S Żegleń, R Przybylski, M ZembalaAnn Transplant 2009; 14(1): 64-65 :: ID: 880435
Abstract
Background: Interstitial fibrosis and variations in cardiocyte hypertrophy belong to the remodelling activities taking place in diseased myocardium especially in dilatative cardiomyopathy (DCM) and ischemic heart disease (IHD). The integrative morphometric measurements should reflect the main differences in histoarchitecture giving a valuable diagnostic tool. We sought to evaluate the quantitative morphology of the explanted diseased heart with DCM and IHD.
Material/Methods: The study groups encompassed 7 hearts with DCM and 8
hearts with IHD explanted during transplantation. The comparative group consisted of myocardial hypertrophy, while the control group were donor fragments. Method. Myocardial histoarchitecture was evaluated morphometrically in histological slides. The cardiocyte hypertrophy was estimated as nucleo-sarcoplasmic ratio calculated from nuclear and cardiocytic integrative measurements; cardiocyte diameter was additionally measured as mean chord, and myocardial interstitial fibrosis - as fibrosis surface area fraction in trichrome stained slides. The results were compared statistically and analyzed with discriminate analysis.
Results: The cardiocyte diameter showed no statistical significance between
myocardial hypertrophy and IHD and IHD and DCM, whereas nucleo-sarcoplasmic ratio showed differences in all comparisons with control groups. Myocardial fibrosis showed no differences between hypertrophy and control. Discriminate analysis pointed out the value of interstitial fibrosis and cardiocytes diameter in differentiating of analyzed groups, then proximity between control and hypertrophy and IHD and DCM and hypertrophy.
Conclusions: Myocardial fibrosis and cardiocytes' diameter may play a role as key parameters for diagnosis and differentiation of cardiomyopathies.
Keywords: Heart Transplantation
In Press
06 May 2022 : Original article
Phosphatidylethanol (PEth) for Monitoring Sobriety in Liver Transplant Candidates: Preliminary Results of D...Ann Transplant In Press; DOI: 10.12659/AOT.936293
27 Apr 2022 : Review article
A Systematic Review of the Literature on Chronic Kidney Disease Following Liver TransplantationAnn Transplant In Press; DOI: 10.12659/AOT.935170
Most Viewed Current Articles
31 Dec 1969 : Original article
Efficacy and Safety of Tacrolimus-Based Maintenance Regimens in De Novo Kidney Transplant Recipients: A Sys...DOI :10.12659/AOT.933588
Ann Transplant 2021; 26:e933588
31 Dec 1969 : Review article
Recurrence of Hepatocellular Carcinoma After Liver Transplantation: Risk Factors and Predictive ModelsDOI :10.12659/AOT.934924
Ann Transplant 2022; 27:e934924
31 Dec 1969 : Review article
Kidney Transplantation in the Times of COVID-19 – A Literature ReviewDOI :10.12659/AOT.925755
Ann Transplant 2020; 25:e925755
31 Dec 1969 : Case report
Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...DOI :10.12659/AOT.935860
Ann Transplant 2022; 27:e935860