Recipient genetic determinants of inï¬‚ammatory process and non-standard atherosclerosis risk factors and their product concentrations at early post transplantation period affect kidney graft function
M Krajewska, K Kościelska-Kasprzak, D Drulis-Fajdasz, W Wejde, K Madziarska, O Mazanowska, M Kusztal, M Klinger
Ann Transplant 2009; 14(1): 38-39
Available online: 2009-05-21
The aim was an analysis of the relations between the recipient's genetic features of selected factors and the twelve month graft function. The relationships between the occurrence of polymorphisms of the genes for MPO, IL-1b, IL-6, CRP, fetuin A, and homocysteine, and gene product concentrations and twelve month transplanted kidney function were analyzed.
Material/Methods: The observation in 125 kidney recipients lasted min. 12; average: 30.9±13.0 months. IL6-174G/C, IL1ÃŸ 3954C/T, MTHFR 677C/T, MTHFR 1298A/C, AHSG 1/2 SNPs were determined with SSP-PCR and MPO-463G/A and CRP-390C/T/A with RLFP analysis. ELISA was applied to mark MPO, fetuin A, IL-6, IL-1ÃŸ and FPIA to mark L-homocysteine concentration.
Results: The highest CRP values were linked with presence of the TT genotype. The positive correlation of CRP concentrations and GFR was observed.
Keywords: Kidney Transplantation