Logo Annals of Transplantation Logo Annals of Transplantation Logo Annals of Transplantation

14 September 2001

Ex-VivoHemoperfusion(eHPS)of Pig-Lungs with Whole Human Blood: Effectsof Complement Inhibitionwith a Soluble CI-Esterase-I nhibitor

H. Schelzig, F. Simon, C. Krischer, A. Vogel, D. Abendroth

Ann Transplant 2001; 6(3): 34-39 :: ID: 497680

Abstract

Objectives: Xenotransplantation could be a future alternative to allotransplantation due to increasing organ shortage. Complement activation plays a major role in hyperacute rejection (HAR) in pig-to-human combinations. We developed an ex-vivo hemoperfusion (EHP) system to investigate pathophysiology of HARin the lung. After standardizing the model, the effect of a soluble complement inhibitor (C I-INH, Berinert'M) was investigated. Methods: Pig lungs were harvested following cold perfusion with Celsior'M preservation solution. EHP was performed using fresh heparinized human blood plus C I-INH (n=6) or heparinized human blood as control (n=4). Bloodgas analyses (BGA), pulmonalarterial pressure (PAP) were monitored. P-selectin and L-selectin were measured. Tissue samples were taken and microscopic changes evall!ated. Results: BGA, PAP, macroscopic and microscopic changes in the control group showed HAR, while the C I-INH group showed significantly longer function. Leucocytes and platelets were markedly activated in the control, wereas in the treated group L-selectin and P-selectin values indicated lower activation. HE stainings showed maintained lung architecture after perfusion of pig-lungs with human blood plus C I-INH. Immunohistochemistry showed less C Iq, C3, CSb-9 activation in the C I-INH group. Conclusions: Investigation of interaction of human blood with pig lung endothelium could be done in this model. C I-INH attenuates HAR in a pig-to-human lung transplantation model by decreasing the activation of adhesion molecules. C I-INH could playa role in induction therapy of future lung xenotransplantation.

Keywords: model, Xenotransplantation, Lung, complement inhibition

0 Comments

In Press

11 Jan 2022 : Original article  

Efficacy of Nitric Oxide-Releasing Nanofibers in Reducing Renal Ischemia-Reperfusion Injury in a Rat Model

Ann Transplant In Press; DOI: 10.12659/AOT.934800  

11 Jan 2022 : Original article  

Effects of Substance P for Liver Regeneration in Rat Hepatectomy Models: A Preliminary Experimental Animal ...

Ann Transplant In Press; DOI: 10.12659/AOT.934801  

Most Viewed Current Articles

29 Dec 2021 : Original article  

Efficacy and Safety of Tacrolimus-Based Maintenance Regimens in De Novo Kidney Transplant Recipients: A Sys...

DOI :10.12659/AOT.933588

Ann Transplant 2021; 26:e933588

22 Dec 2021 : Original article  

Comparison of Coronary Artery Calcium Scoring with Dobutamine Stress Echo for Detection of Coronary Artery ...

DOI :10.12659/AOT.934163

Ann Transplant 2021; 26:e934163

01 Dec 2021 : Original article  

Solid-Phase C1q/C3d Fixing Readouts Correlate with High Median Fluorescence Intensity (MFI) De Novo Donor-S...

DOI :10.12659/AOT.934175

Ann Transplant 2021; 26:e934175

23 Nov 2021 : Original article  

The Future Direction of the Organ Donation System After Legislation of the Life-Sustaining Treatment Decisi...

DOI :10.12659/AOT.934345

Ann Transplant 2021; 26:e934345

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358