14 September 2001
Ex-VivoHemoperfusion(eHPS)of Pig-Lungs with Whole Human Blood: Effectsof Complement Inhibitionwith a Soluble CI-Esterase-I nhibitorH. Schelzig, F. Simon, C. Krischer, A. Vogel, D. Abendroth
Ann Transplant 2001; 6(3): 34-39 :: ID: 497680
Objectives: Xenotransplantation could be a future alternative to allotransplantation due to increasing organ shortage. Complement activation plays a major role in hyperacute rejection (HAR) in pig-to-human combinations. We developed an ex-vivo hemoperfusion (EHP) system to investigate pathophysiology of HARin the lung. After standardizing the model, the effect of a soluble complement inhibitor (C I-INH, Berinert'M) was investigated. Methods: Pig lungs were harvested following cold perfusion with Celsior'M preservation solution. EHP was performed using fresh heparinized human blood plus C I-INH (n=6) or heparinized human blood as control (n=4). Bloodgas analyses (BGA), pulmonalarterial pressure (PAP) were monitored. P-selectin and L-selectin were measured. Tissue samples were taken and microscopic changes evall!ated. Results: BGA, PAP, macroscopic and microscopic changes in the control group showed HAR, while the C I-INH group showed significantly longer function. Leucocytes and platelets were markedly activated in the control, wereas in the treated group L-selectin and P-selectin values indicated lower activation. HE stainings showed maintained lung architecture after perfusion of pig-lungs with human blood plus C I-INH. Immunohistochemistry showed less C Iq, C3, CSb-9 activation in the C I-INH group. Conclusions: Investigation of interaction of human blood with pig lung endothelium could be done in this model. C I-INH attenuates HAR in a pig-to-human lung transplantation model by decreasing the activation of adhesion molecules. C I-INH could playa role in induction therapy of future lung xenotransplantation.
Keywords: model, Xenotransplantation, Lung, complement inhibition
11 Jan 2022 : Original articleEfficacy of Nitric Oxide-Releasing Nanofibers in Reducing Renal Ischemia-Reperfusion Injury in a Rat Model
Ann Transplant In Press; DOI: 10.12659/AOT.934800
Most Viewed Current Articles
29 Dec 2021 : Original articleEfficacy and Safety of Tacrolimus-Based Maintenance Regimens in De Novo Kidney Transplant Recipients: A Sys...
Ann Transplant 2021; 26:e933588
22 Dec 2021 : Original articleComparison of Coronary Artery Calcium Scoring with Dobutamine Stress Echo for Detection of Coronary Artery ...
Ann Transplant 2021; 26:e934163
01 Dec 2021 : Original articleSolid-Phase C1q/C3d Fixing Readouts Correlate with High Median Fluorescence Intensity (MFI) De Novo Donor-S...
Ann Transplant 2021; 26:e934175