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14 September 2001

In VivoMicroscopic Assessment of MicrocirculatoryChanges in a Concordant Xenogeneic Primate Experimental Set Up

Claus Hammer

Ann Transplant 2001; 6(3): 17-24 :: ID: 497579

Abstract

Organ transplantation is always connected with ischemia and thus reperfusion injury of the graft. One of the characteristics in this process is the temporary and permanent adherence of leukocytes to the endothelium of the graft. This cell-to-cell interaction allows the immunocompetent cells to interact in the sense of antigen recognition with mainly defect endothelial cells. It was the aim to study whether induction therapy with poly-clonal ATG' s would reduce or even prohibit these early interactions. Material and methods: The distal extremities of cynomolgus monkeyswere flushedviathe femoralvesselsand reperfused withABO-compatiblehumanheparinisedblood of a hct of30%. Microcirculation was observed applying intra-vital microscopy. The images taken by a CCD-camera are recorded on video tapes for later off line evaluation. pATG I isdirected againstJurkat cells,pATG2 againsthumanthymocytes. Incontrols the blood vesselswere perfused with untreated blood. In groups 2 and 3 the blood was treated with the amount of the ATG .s used in clinical therapy 15 min. prior to perfusion. The total ischemia time was I hour. Results: Five minutes after perfusion rolling was seen in the untreated animal. this increased to change into sticking after 30 min. The blood flow (RBC)in larger venoles remained almost normal. Both olyclonalATG' s inhibitedthe adhesion to a large extend. Conclusion: Ischemia reperfusion results in increasing adherence of leukocytes in the described model. pATG . s suppress this phenomenon completely. This suggests that pATG ' s contain a number of antibodies directed against various types of cell surface molecules which are involved in reperfusion injury and that pATG .s have a favourable influence on the early I/R-mechanisms after organ transplantation and a protective action when used as pre-operative induction therapy.

Keywords: Xenotransplantation, anti-lymphocyte globulin, intra-vital microscopy, adhesion molecules, primate, Microcirculation

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Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358