The Effect of Short G-CSF Administration on the Numbers and Clonogenic Efficiency of Hematopoietic Progenitor Cells in Bone Marrow and Peripheral Blood of Normal Donors
J M Zaucha, W Knopinska-Posluszny, M Bieniaszewska, A Mysliwski, A Hellmann
Ann Transplant 2000; 5(4): 20-26
We have analysed the cellularity, the number of clonogenic cells and their clonogenic efficiency (the number of clonogenic cells/2x IOSMNq in peripheral blood (PB) and bone marrow (BM) during and after filgrastim (rhG-CSF) mobilization of CD34+ cells in 12 healthy donors for allogeneic stem cell donation. G-CSF was administrated subcutaneously for 5 consecutive days at a dose of 10I1g/kg/day.WBC, MNC, CD34+ cell counts, CFU-GM and BFU-E assays in PB were performed at baseline and then daily 12 hours after each G-CSF dose. BMwas assayed before start (day I) and after the last dose (day 6) of G-CSF. Results are given as medians, with ranges in parentheses. In PB the total WBC and MNC increased 7.4-fold (6.0-12.3) and 3.3-fold (1.5-9.4), respectively, reaching a peak of 49.4 x 109/1(32.5-66.6) on day 6 for WBC and 6.28x 109/1(4.7-13.3) for MNC on day 5. CD34+ cell number reached a peak value of 48.0x 106/1(45.6-285) on day 6 whereas CFU-GM and BFU-E reached their peaks on day 5, 0.95 x 104/ml (0.05-6.08) and 1.04x 104/ml, respectively. CFU-MIX, not detectable at baseline, reached a peak of 0.95 x 104/ml (0.006-0.51) on day 5 as well. This was accompanied by an increase in CFU-GM, BFU-E and CFU-MIX clonogenic efficiency: 23-fold (3-150), 9.75-fold (2.2-27.8) and 20-fold (2.5-210), respectively. In BMthe total WBC number increased 2.5-fold (1.3-4.9) from the baseline value of 52.6 x 109/1(7.9-137.0) whereas the MNC count increased 2.0-fold (0.81-3.7) from a baseline of 13.6x 109/1(3.5-54.8). This was, however, not significant. The number of CD34+ cells increased significantly 2.9-fold (0.8-8.3). In 8 donors CFU-MIX were detectable before but not after G-CSF treatment. A similar decrease in CFU-GM and BFU-E clonogenic efficiency occurred but was not significant. CFU-GM and BFU-E numbers did not change. We conclude that the total body numbers of linease committed progenitors increased during G-CSF administration, which indicate their proliferation in addition to mobilization. The effect of G-CSF on the number of more primitive progenitors in BMis less clear and needs further investigation.
Keywords: G-CSF mobilization, Bone Marrow, Stem Cell Transplantation