Monitoring of Human Cytomegalovirus, HHV-6 and HHV-7 Infection in Kidney Transplant Recipients by Molecular Methods to Predict HCMV Disease After Transplantation: A Prospective Study
N Rayes, D Seehofer, S Tullius, A Stein, G May, A Kahl, U Frei, P Neuhaus, H Meisel
Ann Transplant 2005; 10(2): 23-28
Objectives: Recently, highly sensitive molecular assays to detect HCMV, HHV-6 and HHV-7 have been developed but their ability to detect patients at high risk for disease is unclear.
Methods: The positive predictive values (PPV) of pp65-antigenemia, quantitative plasma DNA and pp67-mRNA for CMV-disease were prospectively compared in 82 transplant recipients (72 renal, 10 pancreas-kidney) without CMV-prophylaxis. In addition, the prevalence of HHV- 6 and HHV-7 infection were assessed using qualitative PCR. The assays were performed weekly.
Results: Three patients (3,7%) developed CMV-disease and were effectively treated. They were positive in all three CMV-assays. The PPVs of pp65-Ag, DNA viral load and pp67-mRNA were 33%, 20% and 25% in CMV-positive and 100%, 67% and 50% in seronegative recipients. Sensitivity and negative predictive value were 100% for all assays. Using cut-offs, PPVs were 75% (pp65-Ag ≥20/200.000 cells) and 100% (PCR ≥30.000 copies/ml). Transfusion of >2 packed red cells, rejection and non-functioning graft were risk factors for CMV. Five patients and one patient were positive for HHV-6 and HHV-7 resp.; both were symptomless and did not have a HCMV infection.
Conclusions: Therefore, pp65-antigenemia and plasma PCR with a cut-off could be useful for monitoring preemptive therapy.
Keywords: CMV, Kidney Transplantation, HHV-6, HHV-7, Preemptive Therapy