Logo Annals of Transplantation Logo Annals of Transplantation Logo Annals of Transplantation

15 January 2004 : Original article  

The Biocompatibility of Nitrided Titanium Alloy for Bone Implantation Studied by Laser Flow Cytometry, Laser Scanning Cytometry and Confocal Microscopy

E Czarnowska, A Sowinska, B Cukrowska, M Godlewski, T Wierzchon

Ann Transplant 2004; 9(1A): 72-75 :: ID: 15639


Objectives: Cell adhesion is a fundamental process influencing cell growth and dispersal, and can be studied by different methods.
Methods: The aim of our study was to evaluate the ability of human fibroblasts and osteoblasts to adhere to samples with nitrided surface layers produced under glow discharge conditions on titanium alloy using laser flow cytometry (FC), laser scanning cytometry (LSC) and confocal microscopy (CM). The expression of fibronectin and its receptor – α5β1 integrin (the CD49e molecule) was analyzed.
Results and Conclusions: Our results showed that fibroblasts and osteoblast-like cells (Saos-2 cell lines) adhere to a TiN+Ti2N+αTi(N) surface layer, which favours its application in bone surgery. Employment of LSC together with CM for analysis of cell behavior in contact with biomaterials seems to be very useful, while FC analysis of chemically harvested cells should be carefully taken into account in the study of cell adhesion processes.

Keywords: Nitrided Titanium Alloy, Cell Adhesion, fibronectin, CD49e, Laser Scanning


In Press

06 May 2022 : Original article  

Phosphatidylethanol (PEth) for Monitoring Sobriety in Liver Transplant Candidates: Preliminary Results of D...

Ann Transplant In Press; DOI: 10.12659/AOT.936293  

Most Viewed Current Articles

29 Dec 2021 : Original article  

Efficacy and Safety of Tacrolimus-Based Maintenance Regimens in De Novo Kidney Transplant Recipients: A Sys...

DOI :10.12659/AOT.933588

Ann Transplant 2021; 26:e933588

26 Jan 2022 : Review article  

Recurrence of Hepatocellular Carcinoma After Liver Transplantation: Risk Factors and Predictive Models

DOI :10.12659/AOT.934924

Ann Transplant 2022; 27:e934924

24 Jul 2020 : Review article  

Kidney Transplantation in the Times of COVID-19 – A Literature Review

DOI :10.12659/AOT.925755

Ann Transplant 2020; 25:e925755

15 Mar 2022 : Case report  

Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...

DOI :10.12659/AOT.935860

Ann Transplant 2022; 27:e935860

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358