Logo Annals of Transplantation Logo Annals of Transplantation Logo Annals of Transplantation

15 January 2004 : Original article  

Extracellular Matrix Used in-vitro Model System for Hydroxyapatite Formation

E Pecheva, L Pramatarova, M F Maitz, M T Pham, A Kondyurin

Ann Transplant 2004; 9(1A): 58-60 :: ID: 15635

Abstract

It is generally accepted that bone crystals are nucleated in the interstices of an organic matrix, such as collagen fibrils, but it remains unresolved whether nucleation is activated directly by the matrix or indirectly through non-collagenous proteins absorbed in the hole zones. The aim of this work was to investigate the influence of native extracellular matrix (ECM) proteins, which are known as ligands in reactions with cell surface receptors involved in bone physiology, on the nucleation and growth of hydroxyapatite (HA). The osteoblast-like cell line SAOS-2 is used to synthesize and assemble its own ECM on solid substrates under standard cell culture conditions. After selective removal of cells, a thin film of ECM on the substrates of stainless steel (SS), silicon (S) and silica glass (SG) was obtained. The so-modified surfaces were immersed in a simulated body fluid (SBF), which resembles the ionic composition of human blood plasma. The grown HA layer was studied by XRD, FTIR and Raman spectroscopies, SEM, and EDX.

Keywords: Extracellular Matrix, hydroxyapatite, Stainless Steel, Silicon, Silica Glass

Comments

In Press

06 May 2022 : Original article  

Phosphatidylethanol (PEth) for Monitoring Sobriety in Liver Transplant Candidates: Preliminary Results of D...

Ann Transplant In Press; DOI: 10.12659/AOT.936293  

Most Viewed Current Articles

29 Dec 2021 : Original article  

Efficacy and Safety of Tacrolimus-Based Maintenance Regimens in De Novo Kidney Transplant Recipients: A Sys...

DOI :10.12659/AOT.933588

Ann Transplant 2021; 26:e933588

26 Jan 2022 : Review article  

Recurrence of Hepatocellular Carcinoma After Liver Transplantation: Risk Factors and Predictive Models

DOI :10.12659/AOT.934924

Ann Transplant 2022; 27:e934924

24 Jul 2020 : Review article  

Kidney Transplantation in the Times of COVID-19 – A Literature Review

DOI :10.12659/AOT.925755

Ann Transplant 2020; 25:e925755

15 Mar 2022 : Case report  

Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...

DOI :10.12659/AOT.935860

Ann Transplant 2022; 27:e935860

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358