01 January 2003
Peracetic acid-ethanol treatment of allogeneic avital bone tissue transplants--areliable sterilization method.
Axel Pruss, Ulf B Gobel, Georg Pauli, Moujahed Kao, Michael Seibold, Hans Joachim Monig, Arne Hansen, Rüdiger von VersenAnn Transplant 2003; 8(2): 34-42 :: ID: 142805
Abstract
OBJECTIVES: Based on the European Standard EN 1040, the validation guidelinesof the German Federal Institute for Drugs and Medical Devices and CPMP guidelines we tested the antimicrobialeffectiveness of a peracetic acid-ethanol sterilization procedure (PES) in allogenic avital bone transplants.STUDY DESIGN: Delipidated human bone spongiosa cubes (15 x 15 x 15 mm) served as tissue. Three envelopedviruses (human immunodeficiency virus type 2, pseudorabies virus, bovine virus diarrhoea virus) and threenon-enveloped viruses (hepatitis A virus, poliovirus, porcine parvovirus) were used. The reduction ofvirus infectivity was measured as TCID50/ml in neutralized supernatants and bone homogenates. Staphylococcusaureus. Enterococcus faecium, Pseudomonas aeruginosa. Bacillus subtilis. Clostridium sporogenes, Mycobacteriumterrae. Candida albicans, Aspergillus niger as well as spores of Bacillus subtilis were tested additionally.PES led to a reduction of virus titres by more than 4 log10. Only HAV showed a reduction below 4 log10(2.87) with residual infectivity. After including a delipidating step for HAV-infected cells, a reductionof over 7 log10 HAV titre was found. For viable bacteria, fungi and spores a titre reduction below thedetection level (5 log10) was achieved after an incubation time of 2 hours. CONCLUSIONS: The peraceticacid-ethanol procedure proved to be a reliable method for the sterilization of human bone transplants(layer thickness < or = 15 mm). However, additional safety measures (anamnestic informations, infectiousserology, HIV-/HBV-/HCV-PCR in case of multiorgan donors) should be taken.
In Press
18 Mar 2024 : Original article
Does Antibiotic Use Increase the Risk of Post-Transplantation Diabetes Mellitus? A Retrospective Study of R...Ann Transplant In Press; DOI: 10.12659/AOT.943282
20 Mar 2024 : Original article
Transplant Nephrectomy: A Comparative Study of Timing and Techniques in a Single InstitutionAnn Transplant In Press; DOI: 10.12659/AOT.942252
28 Mar 2024 : Original article
Association Between FEV₁ Decline Rate and Mortality in Long-Term Follow-Up of a 21-Patient Pilot Clinical T...Ann Transplant In Press; DOI: 10.12659/AOT.942823
02 Apr 2024 : Original article
Liver Transplantation from Brain-Dead Donors with Hepatitis B or C in South Korea: A 2014-2020 Korean Organ...Ann Transplant In Press; DOI: 10.12659/AOT.943588
Most Viewed Current Articles
05 Apr 2022 : Original article
Impact of Statins on Hepatocellular Carcinoma Recurrence After Living-Donor Liver TransplantationDOI :10.12659/AOT.935604
Ann Transplant 2022; 27:e935604
12 Jan 2022 : Original article
Risk Factors for Developing BK Virus-Associated Nephropathy: A Single-Center Retrospective Cohort Study of ...DOI :10.12659/AOT.934738
Ann Transplant 2022; 27:e934738
22 Nov 2022 : Original article
Long-Term Effects of Everolimus-Facilitated Tacrolimus Reduction in Living-Donor Liver Transplant Recipient...DOI :10.12659/AOT.937988
Ann Transplant 2022; 27:e937988
15 Mar 2022 : Case report
Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...DOI :10.12659/AOT.935860
Ann Transplant 2022; 27:e935860