15 December 2004
Successful transplantation of hepatocytes requires temporary elimination of scavenger and NK cells, partial hepatectomy and ligation of bile ductA Wesolowska, M Gewartowska, W L Olszewski, M Durlik
Ann Transplant 2004; 9(4): 40-42 :: ID: 13031
Isolated syngeneic and allogeneic hepatocytes transplanted to spleen parenchyma are rapidly disintegrated. Host scavenger monocytes and
macrophages are responsible for this reaction. We designed a method overcoming early disintegration of the intra-splenic grafted hepatocytes. It consisted of administration of anti-asialoGM1 antiserum eliminating NK cells, sublethal whole body irradiation for depletion of scavenging cells and reconstitution with syngeneic bone marrow cells, ligation of host bile duct, intrasplenic hepatocyte transplantation and 3 consecutive partial hepatectomies. Six months after transplantation a glycogen-rich, trabeculae-forming, dividing hepatocytes, situated along strands of newly-formed fibrous tissue and numerous dilated blind bile canaliculae were observed. There was evidently more bile canaliculae in hosts with ligated bile duct than non-ligated controls. This is the first study showing fibrous tissue formed at the site of hepatocyte implantation and stellate cells are presumably responsible for this process.
Keywords: Hepatocytes, bile duct, Transplantation, Scavenger Cells
20 Jan 2022 : Original articleImpact on Waitlist Outcomes from Changes in the Medical Eligibility of Candidates for Simultaneous Liver-Ki...
Ann Transplant In Press; DOI: 10.12659/AOT.934850
19 Jan 2022 : Original articleCarbohydrate Metabolism Disorders in Relation to Cardiac Allograft Vasculopathy (CAV) Intensification in He...
Ann Transplant In Press; DOI: 10.12659/AOT.933420
18 Jan 2022 : Original articlePLGA+Fe₃O₄+PFP Nanoparticles Drug-Delivery Demonstrates Potential Anti-Tumor Effects on Tumor Cells
Ann Transplant In Press; DOI: 10.12659/AOT.933246
Most Viewed Current Articles
29 Dec 2021 : Original articleEfficacy and Safety of Tacrolimus-Based Maintenance Regimens in De Novo Kidney Transplant Recipients: A Sys...
Ann Transplant 2021; 26:e933588
22 Dec 2021 : Original articleComparison of Coronary Artery Calcium Scoring with Dobutamine Stress Echo for Detection of Coronary Artery ...
Ann Transplant 2021; 26:e934163
12 Jan 2022 : Original articleRisk Factors for Developing BK Virus-Associated Nephropathy: A Single-Center Retrospective Cohort Study of ...
Ann Transplant 2022; 27:e934738