Antibodies to HLA Class II Antigens as A Risk Factor for Acute Rejection of The Allogeneic Kidney

We have investigated the association between the presence of antibodies to HLA class II antigens and the development of acute and chronic rejection after kidney transplantation. Sera from seventy-one patients before, shortly (2 weeks), and in the period between 8 and 22 months after transplantation were analyzed by the standard complement-dependent cytotoxicity (CDC) test, ELISA-LATM, and LAT tests. Absence of antibodies to HLA class II antigens before and shortly after transplantation was associated with a lower incidence of rejection episodes in the first post-transplant year. Donor-specific class II antibodies could not be detected by the ELISA-LAT test and there was no statistically significant difference in serum creatinine levels between the antibody-positive and antibody-negative patient groups two years after transplantation. Our study suggests that anti- HLA class II antibodies represent a risk factor for the development of acute immunological complications during the first year after transplantation.

Keywords: Acute Disease, Adult, Antibodies - blood, Enzyme-Linked Immunosorbent Assay - methods, Graft Rejection - epidemiology, Graft Rejection - etiology, Graft Survival, Histocompatibility Antigens Class II - immunology, Incidence, Incidence, Kidney Transplantation, Predictive Value of Tests, Risk Factors, Risk Factors, Survival Analysis, Transplantation, Homologous