14 July 2020: Original Paper
Pretreatment with a Phosphodiesterase-3 Inhibitor, Milrinone, Reduces Hepatic Ischemia-Reperfusion Injury, Minimizing Pericentral Zone-Based Liver and Small Intestinal Injury in Rats
Shinichi Nakanuma 1ABCDEFG* , Hidehiro Tajima 1AC , Hiroyuki Takamura 1ACG , Seisho Sakai 1A , Ryosuke Gabata 1B , Mitsuyoshi Okazaki 1B , Hiroyuki Shinbashi 1B , Yoshinao Ohbatake 1B , Isamu Makino 1B , Hironori Hayashi 2A , Tomoharu Miyashita 1AC , Sachio Fushida 1A , Tetsuo Ohta 1ACEDOI: 10.12659/AOT.922306
Ann Transplant 2020; 25:e922306
Figure 5 Assessment of cleaved caspase-3 expression in the liver parenchyma: The sham group had no cleaved caspase-3-positive cells in the hepatic parenchyma. In the ischemia-reperfusion (IR) group, cleaved caspase-3 was expressed in the cytoplasm of the hepatocytes (black arrows) around the necrotic area (*) in the pericentral zone (zone 3). The IR+milrinone group had fewer cleaved caspase 3-positive hepatocytes (A). The IR group had significantly more cleaved caspase-3-positive hepatocytes in zone 3 than in the periportal zone (zone 1). The IR+milrinone group had significantly fewer cleaved caspase-3-positive hepatocytes in zone 3 than did the IR group (B). * Significantly different (P<0.05) from zone 1 of the IR group. # Significantly different (P<0.05) from the IR group of the same zone. CV – central vein.