Logo Annals of Transplantation Logo Annals of Transplantation Logo Annals of Transplantation

12 November 2015 : Original article  

Induction and Maintenance of Anti-HBs in Immunosuppressed Rats After Liver Transplantation with HBsAg-pulsed Dendritic Cell Complex

Yakun WuE, Yiming LiuB, Jianping GongF, Shengwei LiD, Wei ZhangAC

DOI: 10.12659/AOT.895028

Ann Transplant 2015; 20:676-682

Abstract

BACKGROUND: This study aimed to investigate the efficacy of hepatitis B surface antigen (HBsAg)-pulsed dendritic cell (DC) complex in the induction and maintenance of anti-HBs in immunosuppressed rats after liver transplantation.

MATERIAL AND METHODS: Lewis-Brown Norway (BN) rat liver transplantation models were successfully established. Recipients were injected with tacrolimus (2 mg/Kg) daily post-operation for three months to maintain immunosuppression state; the recipients were then randomly divided into two groups: HBsAg-DC group (n=15) comprised rats intraperitoneally injected with HBsAg-DC complex at 14 and 28 d post-surgery and HBsAg group (n=15) comprised rats injected with HBsAg (200 μL) once a week for 12 weeks. Untreated rats post-transplantation were included in the control group (n=5). Histopathological changes were detected by light microscopy and transmission electron microscopy; mRNA expressions of IL-2 and IFN-γ in graft liver were analyzed through real-time polymerase chain reaction. Serum IL-2 and IFN-γ levels and anti-HB titer were detected through enzyme-linked immunosorbent assay. Changes in CD4+ and CD8+ T cells in the blood were detected through flow cytometry.

RESULTS: IL-2 and IFN-γ expressions were lower in HBsAg-DC and HBsAg groups than in the control group (P<0.05). A high FK506 dose also induced a milder allograft rejection than the control dose. These findings showed that a high FK506 dose caused immunosuppression in rats after liver transplantation. A high anti-HB titer was detected in the HBsAg-DC group in one, two, and three months post-operation; by contrast, anti-HB titer was barely detected in the HBsAg group.

CONCLUSIONS: High anti-HB titers could be induced and maintained in immunosuppressed rats; therefore, HBsAg-DC complex may prevent HBV reinfection after recipients undergo liver transplantation.

Keywords: Hepatitis B Antibodies, Hepatitis B virus, Immunosuppression, Liver Transplantation

Add Comment 0 Comments

In Press

08 Mar 2024 : Original article  

Association of Coronary Calcium Score on Cardiac PET During Pre-Kidney Transplant Assessment with Persisten...

Ann Transplant In Press; DOI: 10.12659/AOT.943532  

14 Mar 2024 : Original article  

Impact of Blood Products Transfusion on Patients in the Immediate Post-Lung Transplant Period: A Cohort Study

Ann Transplant In Press; DOI: 10.12659/AOT.943652  

14 Mar 2024 : Case report  

Treatment of Cavernous Transformation of Portal Vein Caused by Hepatic Cystic Echinococcosis Using Ex Vivo ...

Ann Transplant In Press; DOI: 10.12659/AOT.942358  

15 Mar 2024 : Review article  

Approaches and Challenges in the Current Management of Cytomegalovirus in Transplant Recipients: Highlighti...

Ann Transplant In Press; DOI: 10.12659/AOT.941185  

Most Viewed Current Articles

05 Apr 2022 : Original article  

Impact of Statins on Hepatocellular Carcinoma Recurrence After Living-Donor Liver Transplantation

DOI :10.12659/AOT.935604

Ann Transplant 2022; 27:e935604

12 Jan 2022 : Original article  

Risk Factors for Developing BK Virus-Associated Nephropathy: A Single-Center Retrospective Cohort Study of ...

DOI :10.12659/AOT.934738

Ann Transplant 2022; 27:e934738

22 Nov 2022 : Original article  

Long-Term Effects of Everolimus-Facilitated Tacrolimus Reduction in Living-Donor Liver Transplant Recipient...

DOI :10.12659/AOT.937988

Ann Transplant 2022; 27:e937988

15 Mar 2022 : Case report  

Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...

DOI :10.12659/AOT.935860

Ann Transplant 2022; 27:e935860

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358