Logo Annals of Transplantation Logo Annals of Transplantation Logo Annals of Transplantation

21 May 2009

Donor dependent genetic factors and the long-term renal graft function

M Krajewska, K Kościelska-Kasprzak, D Drulis-Fajdasz, W Weyde, K Madziarska, O Mazanowska, M Kusztal, M Klinger

Ann Transplant 2009; 14(1): 33-33 :: ID: 880318

Abstract

Background: The aim was an analysis of the relations between the genetic  features of fetuin A, homocysteine, PAI-1, MMP-9, MPO, eNOS, IL-1B, IL-6, CRP) in donors and the long-term graft function. The study was extended by an analysis of the impact of the donor's age, gender compatibility, CIT, DGF and the presence of vasculopathy.
Material/Methods: The observation of 125 kidney recipients lasted a min. of twelve months (30.9±13.0 months). Grafts were obtained from 89 donors. CIT was 22.7±6.5 h. AHSG 1/2, MMP9-1562 C/T, IL6-174G/C, IL1b 3954C/T, MTHFR 677C/T, MTHFR 1298A/C, NOS3-786C/T, and PAI1 4G/5G SNPs were determined with the use of SSP-PCR and MPO-463G/A and CRP-390C/T/A with RLFP analysis. NOS3 IV a/b VNTR polymorphism was genotyped through gel electrophoresis of the respective PCR-generated DNA fragment
Results: The presence of the genotype aa eNOS gene was associated with
worse twelve month graft function. The aa genotype was also linked with
acute rejection. The lowest values of GFR after twelve months were achieved by recipients who received a graft with homozygotic PAI1 gene 5G polymorphism, linking paradoxically with lower PAI-1 synthesis suggesting that the intensity of proteolysis leading to an increased alloantigen specificity may stimulate alloresponse. The graft function depended significantly on the donor age and an influence of gender matching was observed. A statistically significant dependence of glomerular fi ltration on DGF was shown. The analysis of histopathological changes confi rmed that the presence of  vasculopathy is a factor of poor prognosis.
Conclusions: The genetic features of the donor influence long-term graft function. Variant eNOS gene polymorphism, which had a functional effect in the form of decreased eNOS activity, was linked with worse remote graft function.  A similar negative impact occurred in the case of donor PAI1 polymorphism, with the functional consequence of lower gene product synthesis

Keywords: Kidney Transplantation

Add Comment 0 Comments

In Press

08 Mar 2024 : Original article  

Association of Coronary Calcium Score on Cardiac PET During Pre-Kidney Transplant Assessment with Persisten...

Ann Transplant In Press; DOI: 10.12659/AOT.943532  

14 Mar 2024 : Original article  

Impact of Blood Products Transfusion on Patients in the Immediate Post-Lung Transplant Period: A Cohort Study

Ann Transplant In Press; DOI: 10.12659/AOT.943652  

14 Mar 2024 : Case report  

Treatment of Cavernous Transformation of Portal Vein Caused by Hepatic Cystic Echinococcosis Using Ex Vivo ...

Ann Transplant In Press; DOI: 10.12659/AOT.942358  

15 Mar 2024 : Review article  

Approaches and Challenges in the Current Management of Cytomegalovirus in Transplant Recipients: Highlighti...

Ann Transplant In Press; DOI: 10.12659/AOT.941185  

Most Viewed Current Articles

05 Apr 2022 : Original article  

Impact of Statins on Hepatocellular Carcinoma Recurrence After Living-Donor Liver Transplantation

DOI :10.12659/AOT.935604

Ann Transplant 2022; 27:e935604

12 Jan 2022 : Original article  

Risk Factors for Developing BK Virus-Associated Nephropathy: A Single-Center Retrospective Cohort Study of ...

DOI :10.12659/AOT.934738

Ann Transplant 2022; 27:e934738

22 Nov 2022 : Original article  

Long-Term Effects of Everolimus-Facilitated Tacrolimus Reduction in Living-Donor Liver Transplant Recipient...

DOI :10.12659/AOT.937988

Ann Transplant 2022; 27:e937988

15 Mar 2022 : Case report  

Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...

DOI :10.12659/AOT.935860

Ann Transplant 2022; 27:e935860

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Annals of Transplantation eISSN: 2329-0358
Annals of Transplantation eISSN: 2329-0358