01 January 2008
Evaluation of the Architect and Dimension tacrolimus and cyclosporine assays
P. Wallemacq, J. S. Gofï¬net, H. Ait-YoucefAnn Transplant 2008; 13(1): 37-38 :: ID: 880197
Abstract
Background: The purpose of this study was to evaluate relative performances of the ARCHITECT and DIMENSION immunoassays, the first requiring preanalytical pretreatment, the last one not.
Material/Methods: Method comparison analyses were performed by testing specimens from transplant recipients. The functional sensitivities (LOQ) of the ARCHITECT and DIMENSION were assessed by testing whole blood specimens spiked with tacrolimus or cyclosporine, run in replicates of 10 on two consecutive days on both instruments. Metabolite cross-reactivity was measured directly in whole blood containing a target concentration of 200 ng/mL cyclosporine and 1,000 ng/mL purified cyclosporine metabolites (AM1, AM1c, AM4N, AM9, AM19). Hematocrit levels were recorded.
Results: The following results were obtained in patient correlation studies: ARCHITECT vs DIMENSION tacrolimus (n=197) Spearman r=0.95, y=0.98x - 0.27 and an average bias of -0.46 ng/mL; ARCHITECT vs DIMENSION cyclosporine (n=95) Spearman r=0.94, y=x - 20 and an average bias of -20.8ng/mL. The LOQ (20%CV) for tacrolimus was 0.6 ng/mL vs 3.1 ng/mL on the ARCHITECT vs DIMENSION, respectively. For cyclosporine these values were 12.1 ng/mL vs 28 ng/mL, respectively for ARCHITECT vs Dimension. Statistical analysis of the 3 ng/mL functional sensitivity panel yielded the following results: ARCHITECT, mean =3.03 ng/mL (95% CI=2.71-3.36); DIMENSION, mean =2.80 ng/mL (95% CI=1.44-4.15). Cyclosporine metabolite cross-reactivity ranged from -0.8% up to 2.2% for the ARCHITECT and from -0.1 up to 6.4% for the DIMENSION, respectively. Hematocrit did not affect any of these assays.
Conclusions: Both assays displayed satisfactory analytical performances. However, from these data, it is suggested that the preanalytical steps involved in the ARCHITECT as says might contribute positively in their analytical performances (particularly the LOQ) as compared to the fully automated DIMENSION.
Keywords: -----, bladder tissue, electrical impedance, first record-able reading, optimal probe pressure, Tacrolimus
In Press
08 Mar 2024 : Original article
Association of Coronary Calcium Score on Cardiac PET During Pre-Kidney Transplant Assessment with Persisten...Ann Transplant In Press; DOI: 10.12659/AOT.943532
14 Mar 2024 : Original article
Impact of Blood Products Transfusion on Patients in the Immediate Post-Lung Transplant Period: A Cohort StudyAnn Transplant In Press; DOI: 10.12659/AOT.943652
14 Mar 2024 : Case report
Treatment of Cavernous Transformation of Portal Vein Caused by Hepatic Cystic Echinococcosis Using Ex Vivo ...Ann Transplant In Press; DOI: 10.12659/AOT.942358
15 Mar 2024 : Review article
Approaches and Challenges in the Current Management of Cytomegalovirus in Transplant Recipients: Highlighti...Ann Transplant In Press; DOI: 10.12659/AOT.941185
Most Viewed Current Articles
05 Apr 2022 : Original article
Impact of Statins on Hepatocellular Carcinoma Recurrence After Living-Donor Liver TransplantationDOI :10.12659/AOT.935604
Ann Transplant 2022; 27:e935604
12 Jan 2022 : Original article
Risk Factors for Developing BK Virus-Associated Nephropathy: A Single-Center Retrospective Cohort Study of ...DOI :10.12659/AOT.934738
Ann Transplant 2022; 27:e934738
22 Nov 2022 : Original article
Long-Term Effects of Everolimus-Facilitated Tacrolimus Reduction in Living-Donor Liver Transplant Recipient...DOI :10.12659/AOT.937988
Ann Transplant 2022; 27:e937988
15 Mar 2022 : Case report
Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...DOI :10.12659/AOT.935860
Ann Transplant 2022; 27:e935860