01 January 2008
Evaluation of the Architect and Dimension tacrolimus and cyclosporine assays
P. Wallemacq, J. S. Gofï¬net, H. Ait-YoucefAnn Transplant 2008; 13(1): 37-38 :: ID: 880197
Abstract
Background: The purpose of this study was to evaluate relative performances of the ARCHITECT and DIMENSION immunoassays, the first requiring preanalytical pretreatment, the last one not.
Material/Methods: Method comparison analyses were performed by testing specimens from transplant recipients. The functional sensitivities (LOQ) of the ARCHITECT and DIMENSION were assessed by testing whole blood specimens spiked with tacrolimus or cyclosporine, run in replicates of 10 on two consecutive days on both instruments. Metabolite cross-reactivity was measured directly in whole blood containing a target concentration of 200 ng/mL cyclosporine and 1,000 ng/mL purified cyclosporine metabolites (AM1, AM1c, AM4N, AM9, AM19). Hematocrit levels were recorded.
Results: The following results were obtained in patient correlation studies: ARCHITECT vs DIMENSION tacrolimus (n=197) Spearman r=0.95, y=0.98x - 0.27 and an average bias of -0.46 ng/mL; ARCHITECT vs DIMENSION cyclosporine (n=95) Spearman r=0.94, y=x - 20 and an average bias of -20.8ng/mL. The LOQ (20%CV) for tacrolimus was 0.6 ng/mL vs 3.1 ng/mL on the ARCHITECT vs DIMENSION, respectively. For cyclosporine these values were 12.1 ng/mL vs 28 ng/mL, respectively for ARCHITECT vs Dimension. Statistical analysis of the 3 ng/mL functional sensitivity panel yielded the following results: ARCHITECT, mean =3.03 ng/mL (95% CI=2.71-3.36); DIMENSION, mean =2.80 ng/mL (95% CI=1.44-4.15). Cyclosporine metabolite cross-reactivity ranged from -0.8% up to 2.2% for the ARCHITECT and from -0.1 up to 6.4% for the DIMENSION, respectively. Hematocrit did not affect any of these assays.
Conclusions: Both assays displayed satisfactory analytical performances. However, from these data, it is suggested that the preanalytical steps involved in the ARCHITECT as says might contribute positively in their analytical performances (particularly the LOQ) as compared to the fully automated DIMENSION.
Keywords: -----, bladder tissue, electrical impedance, first record-able reading, optimal probe pressure, Tacrolimus
In Press
18 Mar 2024 : Original article
Does Antibiotic Use Increase the Risk of Post-Transplantation Diabetes Mellitus? A Retrospective Study of R...Ann Transplant In Press; DOI: 10.12659/AOT.943282
20 Mar 2024 : Original article
Transplant Nephrectomy: A Comparative Study of Timing and Techniques in a Single InstitutionAnn Transplant In Press; DOI: 10.12659/AOT.942252
28 Mar 2024 : Original article
Association Between FEV₁ Decline Rate and Mortality in Long-Term Follow-Up of a 21-Patient Pilot Clinical T...Ann Transplant In Press; DOI: 10.12659/AOT.942823
02 Apr 2024 : Original article
Liver Transplantation from Brain-Dead Donors with Hepatitis B or C in South Korea: A 2014-2020 Korean Organ...Ann Transplant In Press; DOI: 10.12659/AOT.943588
Most Viewed Current Articles
05 Apr 2022 : Original article
Impact of Statins on Hepatocellular Carcinoma Recurrence After Living-Donor Liver TransplantationDOI :10.12659/AOT.935604
Ann Transplant 2022; 27:e935604
12 Jan 2022 : Original article
Risk Factors for Developing BK Virus-Associated Nephropathy: A Single-Center Retrospective Cohort Study of ...DOI :10.12659/AOT.934738
Ann Transplant 2022; 27:e934738
22 Nov 2022 : Original article
Long-Term Effects of Everolimus-Facilitated Tacrolimus Reduction in Living-Donor Liver Transplant Recipient...DOI :10.12659/AOT.937988
Ann Transplant 2022; 27:e937988
15 Mar 2022 : Case report
Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem...DOI :10.12659/AOT.935860
Ann Transplant 2022; 27:e935860